作者：Jin Zhang, Lishuan Wu, Wei Fan,
Xiaoling Zhang, Huixia Jia, Yu Li, Yafang Yin, Jianjun Hu*, Mengzhu Lu.
期刊：Frontiers in Plant Science
发表时间：2015年
卷.期.页码：6:548
摘要：Proteomic analysis was used to generate a map of Populus
deltoides CL. “2KEN8” mature pollen proteins. By applying 2-D
electrophoresis, we resolved 403 protein spots from mature pollen. Using the
matrix-assisted laser desorption/ionization time time-of-flight/time-of-flight
tandem mass spectrometry method, we identified 178 distinct proteins from 218
protein spots expressed in mature pollen. Moreover, out of these, 28 proteins
were identified as putative allergens. The expression patterns of these putative
allergen genes indicate that several of these genes are highly expressed in
pollen. In addition, the members of profilin allergen family were analyzed and
their expression patterns were compared with their homologous genes in Arabidopsis
and rice. Knowledge of these identified allergens has the potential to improve
specific diagnosis and allergen immunotherapy treatment for patients with
poplar pollen allergy.
